Proteomic Technologies
The core is already well equipped with a broad array of state-of-the-art
analytical instrumentation for proteomic studies, which include
- ABI 4700 MALDI-TOF/TOF mass spectrometer
- Ciphergen SELDI-TOF
mass spectrometer
- Agilent 1100 Series LC/MSD mass spectrometer
- Detailed protein structural characterization
using tandem mass spectrometry in microcapillary LC/MS/MS mode
to fully characterize potential target proteins for sequence
and post-translational modifications with lower throughput
- ABI QSTAR Pulsar Hybrid mass spectrometer for LC/MS/MS
- Electro spray MS by Thermo Finnigan LCQ Deca Ion Trap Mass Spectrometer for
LC/MS/MS
- Beckman PF2D two-dimensional
liquid chromatography system for protein fractionation
- Agilent
1100 Series Liquid chromatography system
- High resolution LC by Dionex Nano LC Dual Gradient Capillary
Separation System
- Beckman Biomek
FX Robotic system
- Amerhsam Lucidea arrayer for protein and
peptide microarrays
- Amersham ASP automatic hybridization station
- Biomicrosystems
Maui automatic hybridization station
- Perkin Elmer ScanArray Express HT
Laser Scanner
The instrumentation will be operated by the highly trained technical
staff of the core laboratory. The core has also developed standard
procedures for quality control to assure the timely delivery
of accurate and high quality data. The core will implement procedures
for the proper handling, processing and storage of proteomic samples.
Bioinformatics Core
The Bioinformatics Core will be the central portal of the Proteomics
Core that will provide DF/HCC investigators with access to phenotypically
annotated proteomics data as well as a large set of analytic procedures
(i.e. unsupervised and supervised learning techniques, statistical analysis
tools, automated clustering and classification procedures, pathway analysis
and generation tools, protein identification database tools, data-visualization
tools, annotation tools) with which to explore the data sets for further
hypothesis generation.
Finally, a consultative service provided by key members of the core
will be available at all times for DF/HCC investigators to assess the
rationale and feasibility for undertaking specific projects, designing
proteomic studies, process and protocol development, proteomic data analysis,
and the interpretation of results.
